Advanced Time-Correlated Single Photon Counting Techniques

This comprehensive guide explores the sophisticated world of time-correlated single photon counting, building upon the foundational 1984 work by O'Connor and Phillips while addressing the dramatic technological evolution this measurement technique has undergone. Where early TCSPC was primarily confined to recording fluorescence decays in controlled cuvette environments, this text delves into the advanced methodologies that now enable vastly faster acquisition times and more complex applications. It systematically details how modern high-repetition-rate lasers and high-speed electronics have transformed TCSPC from a powerful but slow laboratory tool into a dynamic technique capable of tackling real-time scientific challenges.

The book's strength lies in its authoritative explanation of how these technical advancements unlock new experimental possibilities, particularly for researchers pushing the boundaries of biophysics, materials science, and single-molecule spectroscopy. Readers will gain crucial insights into optimizing TCSPC systems for maximum sensitivity and temporal resolution, moving far beyond basic decay curve analysis. For scientists and engineers working with cutting-edge photonic instrumentation, this resource provides the essential theoretical and practical knowledge to fully leverage modern TCSPC's capabilities in their own pioneering work.